Interference in Macrophage Balance (M1/M2): The Mechanism of Action Responsible for the Anti-Inflammatory Effect of a Fluorophenyl-Substituted Imidazole

Figure 4

Effect of fluorophenyl-imidazole on iNOS (a); COX-2 (b); ARG-1 (c); and FIZZ-1 (d) mRNA expression in RAW 264.7 macrophages stimulated with LPS (1 μg/mL). The experimental groups used was B: cells pretreated with vehicle and incubated with PBS (blank group); LPS: cells pretreated with vehicle and stimulated with LPS (1 μg/mL); dexa: cells pretreated with dexamethasone (7 μM) and stimulated with LPS (1 μg/mL); flu: fluorophenyl-imidazole: cells pretreated with the best concentration (based on IC₅₀ values) (1 µM) of fluorophenyl-imidazole alone, and flu (1 μM) + LPS: cells pretreated with the best concentration (based on IC₅₀ values) (1 µM) of fluorophenyl-imidazole and stimulated with LPS (1 μg/mL). iNOS, COX-2, ARG-1, and FIZZ-1 mRNA were calculated in duplicate by relative CT method in comparison to the (S) group results (2^−ΔΔCT). The results were expressed as the mean ± SEM; n = 3; ; and .

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