Assessment of Intratumoral Doxorubicin Penetration after Mild Hyperthermia-Mediated Release from Thermosensitive Liposomes

Figure 5

Modeling of released-DOX penetration in the tumor microenvironment. The implemented evolution model allowed calculating a drug diffusion of ν = 2500 µm²·s⁻¹ (e–h). Only the background fluorescence served in the evolution model, as shown by the absolute difference maps (i–l) calculated between the experimental data and the computed released-DOX penetration by the evolution model. The parameters to apply were calculated for the released-DOX penetration model (i.e., equation (4)). The x-axis reports regularly sampled values of the diffusion parameter ν that are exhaustively enumerated during the optimization process. (m) The optimization of the released-DOX apparent diffusion parameter ν: the averaged least-square residue between the model and the measured data was minimal for ν = 2500 µm²·s⁻¹. Optimal released-DOX deposition δ and vascular washout ω are displayed for each value of ν in (n) and (o), respectively.