Evaluation of Myelin Radiotracers in the Lysolecithin Rat Model of Focal Demyelination: Beware of Pitfalls!

Figure 4

Ex vivo autoradiography with [¹⁸F]AV-45. Reduced [¹⁸F]AV-45 uptake in the LPC-injected site was visually identified ((a), arrow), and matched T2WI MRI (b), as well as Sudan Black B staining, which confirmed demyelination (c). Four sections per animal (each rat is represented with a different color), encompassing the whole area showing a decreased binding, were analyzed, hence resulting in 20 measurements (d). There was a significant correlation (r = 0.559, ) between the ratio of ipsi-to-contralateral [¹⁸F]AV-45 binding on autoradiography (ARG) and the corresponding ratio of ipsi-to-contralateral optical density on Sudan Black B (SBB) staining (plain line, linear fit; dashed lines, 95% confidence interval).

(a)

(b)

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