lncRNA SNHG12 Inhibition Based on Microsystem Cell Imaging Technology Protects the Endothelium from LPS-Induced Inflammation by Inhibiting the Expression of miR-140-3p Target Gene fndc5

Figure 3

SNHG12 target validation. LncBASE2.1 was used to predict binding targets (a), while the interaction was verified through luciferase reporter gene assay (b). The result of qRT-PCR showed the expression level of miR-140-3p (c). Apoptosis level was detected by flow cytometry (d). In addition, ELISA was used to detect inflammatory cytokines in cell supernatant (e). ROS levels were detected by ROS kits (f). Finally, the cell permeability experiment was performed (g). ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)