lncRNA SNHG12 Inhibition Based on Microsystem Cell Imaging Technology Protects the Endothelium from LPS-Induced Inflammation by Inhibiting the Expression of miR-140-3p Target Gene fndc5

Figure 4

The downstream target of miR-140-3p is fndc5. fndc5 was estimated as miR-140-3p′s target (a). Luciferase report confirmed the targeted combination of fndc5 and miR-140-3p and the direct interaction between the two molecules (b). The fndc5 level was detected by qRT-PCR (c). Apoptosis level was detected by flow cytometry (d). Inflammatory cytokine expression within supernatant was analyzed via ELISA (e). ROS levels were detected (f). Cell permeability test (g). ; .

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