In silico Prediction of Deleterious Single Nucleotide Polymorphism in S100A4 Metastatic Gene: Potential Early Diagnostic Marker

Table 4

Detection of SNPs effect in the S100A4 regulatory region by PolymiRTS database.

Location	dbSNP ID	Variant type	Wobble base pair	Ancestral	Allele	miR ID	Conservation	miRSite	Function	Experimental	Context + score change
				Allele					Class	Support
153516154	rs113443697	SNP	N	G	G	hsa-miR-505-5p	7	acccTGGCTCCtt	D	N	−0.217
					C	hsa-miR-1207-5p	5	aCCCTGCCtcctt	C	N	−0.255
						hsa-miR-1827	5	accCTGCCTCctt	C	N	−0.169
						hsa-miR-3612	7	acccTGCCTCCtt	C	N	−0.216
						hsa-miR-4695-5p	5	accctGCCTCCTt	C	N	−0.238
						hsa-miR-4763-3p	5	aCCCTGCCtcctt	C	N	−0.255
						hsa-miR-650	7	acccTGCCTCCtt	C	N	−0.16
						hsa-miR-6808-5p	5	acCCTGCCTcctt	C	N	−0.189
						hsa-miR-6893-5p	5	acCCTGCCTcctt	C	N	−0.17
						hsa-miR-940	5	acCCTGCCTcctt	C	N	−0.133
153516160	rs1051044	SNP	N	A	C	hsa-miR-6798-5p	6	TtttcCCCCCTGg	C	N	−0.226
153516185	rs143742855	SNP	N	C	C	hsa-miR-1273a	2	cccTGTCGCCAgt	D	N	−0.437
					T	hsa-miR-196a-3p	2	cccTGTTGCCAgt	C	N	−0.264
					T	hsa-miR-3128	2	ccctgTTGCCAGt	C	N	−0.093

N in wobble pair column represents ‘No.’ This indicates that the particular SNP cannot form a G:U wobble base pair with miRNA. N in the experimental support column also represents ‘No’ It indicates that so far no experimental data have been reported for the predicted target site. In the functional class column, D represents that a derived allele disrupts a conserved miRNA site (ancestral allele with support ≥2), and N represents that the derived allele disrupts a nonconserved miRNA site (ancestral allele with support ≥2).