[Retracted] IGF-1 Promotes Epithelial-Mesenchymal Transition of Lens Epithelial Cells That Is Conferred by miR-3666 Loss

Figure 2

Overexpression of IGF-1 results in EMT in HLECs. (a–c) qRT-PCR was used to confirm mRNA expression of IGF-1, CDH1, and vimentin to detect the effect of IGF-1 overexpression in HLECs, n = 6. (d) The protein levels of IGF-1, E-cadherin, and vimentin were determined by western blot to detect the effect of IGF-1 overexpression in HLECs. (e) Analysis of western blot, n = 4. (f) The wound-healing assay revealed that overexpression of IGF-1 increased cell migration in HLECs. (g) Analysis of wound-healing assay, n = 6. (h) Transwell assay showed the invasion ability of HLECs was promoted by IGF-1. (i) Analysis of Transwell assay, n = 6. (j) EdU staining illustrated that forced expression of IGF-1 promoted cell proliferation. (k) Analysis of EdU staining, n = 6. ; .

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