[Retracted] IGF-1 Promotes Epithelial-Mesenchymal Transition of Lens Epithelial Cells That Is Conferred by miR-3666 Loss

Figure 3

Inhibition of IGF-1 ameliorated HLEC-EMT after TGF-β2 was treated. (a) qRT-PCR was used to verify the efficiency of sh-IGF-1, n = 6. (b, c) Silencing IGF-1 reversed TGF-β2-induced mRNA expression of CDH1 and vimentin, which was measured by qRT-PCR assay. (d) Using western blot to investigate the effect of sh-IGF-1 with or without TGF-β2 on the level of IGF-1, E-cadherin, and vimentin proteins. (e) Analysis of western blot, n = 4. (f) Wound healing revealed that inhibiting IGF-1 ablated the cell migration induced by TGF-β2. (g) Analysis of wound healing assay, n = 6. (h) Transwell assay showed that invasion ability of HLECs was inhibited by sh-IGF-1. (i) Analysis of Transwell assay, n = 6. (j) EdU staining revealed that knockdown of IGF-1 inhibited cell proliferation induced by TGF-β2. (k) Analysis of EdU staining, n = 6. ; .

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