[Retracted] IGF-1 Promotes Epithelial-Mesenchymal Transition of Lens Epithelial Cells That Is Conferred by miR-3666 Loss

Figure 5

Overexpression of miR-3666 attenuated TGF-β2-induced EMT in HLECs. (a, b) miR-3666 mimics reversed TGF-β2-induced mRNA expression of CDH1 and vimentin, which was measured by qRT-PCR. (d) Using western blot to investigate the effect of miR-3666 mimics with or without TGF-β2 on the level of IGF-1, E-cadherin, and vimentin proteins. (e) Analysis of western blot, n = 4. (f) Wound healing revealed that miR-3666 mimics ablated the increased cell migration induced by TGF-β2. (g) Analysis of wound-healing assay, n = 6. (h) Transwell assay showed that the invasion ability of HLECs was inhibited by miR-3666 mimics. (i) Analysis of Transwell assay, n = 6. (j) EdU staining illustrated that miR-3666 mimics inhibited cell proliferation induced by TGF-β2.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)