Protective Effect of Galangin Methylation Modification Based on Cell Imaging on Inflammatory Lung Injury and Its Molecular Mechanism

Table 1

Sequences related to amplified IL-6 and TNF-a promoter regions (including TF binding sites) and the reference sequence.

	Position (distance from TSS)	Sequence information	PCR primer
IL-6 promotor	−632 ⟶ −358	aaggagacacgagacgaag........	F:5′-aaggagacacgagacgaag-3′
		p65
		..............gtattgtgtacgttgctta	R:5′-attcgttgcatgtgtatg-3′
		p65
TNF-a promotor	−656 ⟶ −319	aagcgaattctcagacgcagcca........	F:5′-ctccattctcagacgcagcca-3′
		AP-1
		......gtctaagtctcccgcctac	R:5′-catccgccctcgtctaagt-3′
		AP-1
Reference sequence (IL-6 intron)	74 ⟶ 231	tccattcattctctttgctcctg...............	F:5′-tccattcattctctttgct-3′
		.............tctattcacagtggacacaatta	R:5′-ttcacagtggacacaatta-3′

Note. Bold parts indicate the TF binding sites.