Ketamine Promotes LPS-Induced Pulmonary Autophagy and Reduces Apoptosis through the AMPK/mTOR Pathway

Figure 1

LPS treatment induced acute lung injury in mice. (a) The dry-wet weight ratio of the ALI + ketamine group was obviously lower than that of the ALI group. (b)–(d) ELISA assay was used to detect changes in IL-1β, IL-6 and TNF-α in lung tissues. Ketamine significantly reduced the levels of IL-1β, IL-6 and TNF-α. (e) and (f) qRT-PCR was used to detect changes in Bax, Bcl-2, Beclin-1 and p62 expression levels in lung tissues. Results showed that mRNA expressions of Bax and P62 were lower than those of the ALI group, while the mRNA expressions of Bcl-2 and Beclin-1 were higher than those of the ALI group. (“” indicates that compared with the Sham group, “#” indicates that compared with ALI group ).

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