Regulating the Wnt/β-catenin Signaling Pathway Promotes Repigmentation in Vitiligo Using Fire Needle Therapy

Figure 2

Development of the vitiligo mouse model and the expression of related genes. (a) Immunization schedule. C57BL/6J mice were immunized subcutaneously twice at a one-week interval into the hind footpad with TRP2-180, LPS and CpG ODN. One week after the same adjuvants were injected twice intradermally into the tail, again at a one-week interval (Figure 2(a)). After four weeks of final immunization, we observed depigmented skin lesions around the tail injection site. (b) Developed depigmented skin lesions in the tail at week seven. (c) Representative pictures show haematoxylin and eosin staining in the tail sections of the control and vitiligo mouse model (Scale bar = 100 μm). (d) The mRNA expression levels of Trp1, Trp2, Tyr, Mitf, Wnt3, Wnt3a, Wnt10b, Lef1, Ctnnb1 in the vitiligo model mouse skin and control mouse skin by qRT-PCR. Data are mean ± SEM. , , , significant differences are indicated. ns no significant difference.