Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage

Table 1

World Health Organization (WHO) designated variants of concern (VOC) [11].

WHO label	Alpha	Beta	Gamma	Delta	Omicron
Pango lineage	B.1.1.7	B.1.351	P.1	B.1.617.2	B.1.1.529
Classifying Mutation(s)	Δ69/70	K417N	K417N/T	T19R	A67V	E484A
	Δ144Y	E484K	E484K	(G142D)	Δ69-70	Q493K
	(E484K)	N501Y	N501Y	Δ156	T95I	G496S
	(S494P)	D614G	D614G	Δ157	G142D	Q498R
	N501Y			R158G	Δ143–145	N501Y
	A570D			L452R	Δ211	Y505H
	D614G			T478K	L212I	T547K
	P681H			D614G	ins214EPE	D614G
				P681R	G339D	H655Y
				D950N	S371L	N679K
					S373P	P681H
					S375F	N764K
					K417N	D796Y
					N440K	N856K
					G446S	Q954H
					S477N	N969K
					T478K	L981F

Detected in some sequences but not all.