Transcriptome Profiles of the Liver in Two Cold-Exposed Sheep Breeds Revealed Different Mechanisms and Candidate Genes for Thermogenesis

Figure 4

Expression levels of four candidate genes from RT-PCR and RNA-seq. A-liver^w and A-liver^c: liver of Altay lambs under thermoneutral (20°C; n = 4) and cold exposure (−5°C; n = 5); H-liver^w and H-liver^c: liver of Hu lambs under thermoneutral (20°C; n = 4) and cold exposure (−5°C) (n = 6). The X-axis represents the tissue of the liver; the Y-axis on the left represents the relative gene FPKM levels of RNA-seq by columns and bars; the Y-axis on the right represents the relative gene expression levels of RT-PCR by columns and bars. ^∗P < 0.05.