In Silico Analysis of the Gene Expression Patterns between Aldosterone-Producing Adenoma and Nonfunctional Adrenocortical Adenoma

Figure 2

WGCNA analysis of combined datasets. (a) Scale independence and mean network connectivity for soft thresholding powers (β). β = 6 was selected to achieve model fit maximization. (b) In total, 22,848 genes were assigned to 19 modules, including one gray module, with cutoff of powers = 6. The top image shows a gene dendrogram, and the bottom image shows the gene modules with different colors. (c) Correlation between modules and traits. The upper number in each cell refers to the correlation coefficient of the corresponding module with clinical trait, and the lower number is the corresponding value. (d) The eigengene dendrogram and heat map. The 19 modules are relatively independent, and the brown module was strongly correlated with APA. (e), (f) Scatter plots of GS versus MM for APA in the brown and tan modules. (g) Topological overlap matrix heatmap of 400 genes selected from a total of 22,848 genes. Light color represents high overlap of the genes, and progressively darker color indicates lower overlap. Blocks of light colors along the diagonal represent the coexpression modules.

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