Genetic Analysis of Prunus salicina L. by Random Amplified Polymorphic DNA (RAPD) and Intersimple Sequence Repeat (ISSR)
Table 3
The characteristics of the bands generated by RAPD primers.
Primer
TF
PF
PFR (%)
PIC
RP
EMR
MI
S7
19
15
78.95
0.33
25.48
11.84
3.90
S17
18
16
88.89
0.46
19.30
14.22
6.60
S21
16
14
87.50
0.36
20.43
12.25
4.43
S43
21
18
85.71
0.45
23.13
15.43
6.93
S58
14
12
85.71
0.20
22.43
10.29
2.04
S121
14
12
85.71
0.34
18.52
10.29
3.48
S160
18
12
66.67
0.38
22.35
8.00
3.03
S201
20
13
65.00
0.30
28.17
8.45
2.50
S256
15
13
86.67
0.41
17.57
11.27
4.67
S412
15
13
86.67
0.34
19.65
11.27
3.89
S1403
19
13
68.42
0.36
24.43
8.89
3.18
S1409
18
15
83.33
0.39
21.83
12.50
4.92
SBS-A16
14
12
85.71
0.34
18.61
10.29
3.45
OPA-4
10
7
70.00
0.24
15.13
4.90
1.19
OPA-10
19
18
94.74
0.49
19.22
17.05
8.43
OPB-8
15
13
86.67
0.38
18.52
11.27
4.31
RAPD-1
16
13
81.25
0.40
19.04
10.56
4.28
RAPD-5
15
11
73.33
0.37
18.87
8.07
2.99
RAPD-7
19
17
89.47
0.37
24.00
15.21
5.60
Average
16.58
13.53
81.60a
0.36a
20.88a
11.16a
4.20a
Minimum
10
7
65.00
0.20
15.13
4.90
1.19
Maximum
21
18
94.74
0.49
28.17
17.05
8.43
Total
315
257
Note. aAverage of the column. TF, total number of fragments; PF, number of polymorphic fragments; PFR, polymorphic fragments ratios (%); PIC, polymorphism information content; RP, resolving power; EMR, effective multiplex ratio; MI, marker index.