Chloroplast DNA Diversity of Tunisian Barley Landraces as Revealed by cpSSRs Molecular Markers and Implication for Conservation Strategies

Table 2

Characteristics of the six cpSSR (chloroplast microsatellites) molecular markers and genetic diversity parameters across the 60 studied barley landraces. Tm: primer melting temperature, Na: number of alleles per locus, Ne: number of effective alleles per locus, I : Shannon’s information index, h: genetic diversity, uh: unbiased genetic diversity.

Locus	Repeats	Tm°C	Na	Ne	I	h	Uh
hvcppsbA	(T)8	50	2	1.800	0.637	0.444	0.452
hvcprpoA	(T)8(CTT)3	60	2	2.000	0.693	0.500	0.508
hvcprps12	(T)8	60	2	1.557	0.543	0.358	0.364
hvcptrnS1	(A)7CGC(T)13	60	2	1.220	0.325	0.180	0.183
hvcptrnS2	(T)10	60	3	2.410	0.960	0.585	0.595
hvcptrnLF	(C)9	60	2	1.897	0.666	0.473	0.481
Means			2.167	1.814	0.637	0.423	0.431
SD			0.167	0.165	0.084	0.057	0.058