LncRNA HOXA-AS2 Promotes Temozolomide Resistance in Glioblastoma by Regulated miR-302a-3p/IGF1 Axis

Figure 3

Knockdown of HOXA-AS2 results in miR-302a-3p upregulation and GBM cell sensitization to TMZ. (a) HOXA-AS2 was identified as a putative ceRNA for miR-302a-3p. (b) Luciferase reporter assays were conducted by transfecting HEK293T cells using miR-302a-3p and WT or mutated (MUT) HOXA-AS2 reporter vectors. (c) HEK293T cells were utilized to conduct an anti-AGO2 RIP assay, after which miR-302a-3p and HOXA-AS2 levels in precipitated samples were detected via qPCR. (d) miR-302a-3p levels were detected in GBMTR cells. (e) Changes in miR-302a-3p expression following HOXA-AS2 knockdown were examined in GBMTR cells via qPCR. (f) Levels of miR-302a-3p were assessed in TMZ-resistant (n = 132) and sensitive (n = 132) GBM patient tissues. (g) HOXA-AS2 and miR-302a-3p levels in TMZ-resistant GBM tissue samples were negatively correlated. (h) Following miR-302a-3p inhibitor or control construct transfection, levels of this miRNA were detected via qPCR in GBMTR cells. (i) Following exposure to the indicated TMZ doses, GBMTR cell viability and corresponding IC50 values were detected via CCK-8 assay. .

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