Positive Expression of Retinol-Binding Protein 4 Is Related to the Malignant Clinical Features Leading to Poor Prognosis of Glioblastoma

Figure 3

RBP4 promotes GBM proliferation, migration, and invasion. (a) LN229 and U87 cells were transfected with pcDNA3.0-RBP4 plasmids (overexpress), transfection reagent (control), or RBP4-siRNA, respectively. The transfection efficiencies were tested by western blot. (b) Proliferation of transfected LN229 and U87 cells were tested via MTT method. The proliferation curves indicated that overexpressing RBP4 promotes cell proliferation while silencing RBP4 inhibits cell proliferation. (c) Colony formation assays were also conducted to evaluate cell proliferation capacity. Comparing with control cells, clone-forming capacities of RBP4-overexpressed cells were significantly enhanced while impaired in RBP4-knockdown cells. (d) Migration capacities of transfected cells were tested by Transwell assay, which demonstrated that RBP4 can positively regulation the migration process. (e) Invasion abilities of GBM cells were assessed by Matrigel–Transwell method. Accordingly, overexpressing RBP4 increased the number of invaded cells while silencing RBP4 decreased the number of invaded cells. Data were presented as mean ± SD. Each experiment was conducted for three independent times. compared to control group.

(a)

(b)

(c)

(d)

(e)