Sirt3 Regulates Response to Oxidative Stress by Interacting with BER Proteins in Colorectal Cancer

Figure 2

Coimmunoprecipitation of Sirt3 with MUTYH, NEIL1, and APE1 proteins and lack of coimmunoprecipitation with NEIL2 and LIG3. Coimmunoprecipitation section shows the presence or absence of physical interaction after incubation with antibodies against the indicated protein (with IgG as negative control) followed by incubation with anti-Sirt3. Input section shows the presence of protein after treatment with indicated antibody without coimmunoprecipitation.