Genetics Research

Research Article

Sirt3 Regulates Response to Oxidative Stress by Interacting with BER Proteins in Colorectal Cancer

Table 1

Effect of Sirt3 gene silencing and overexpression on the levels of Sirt3, APE1, NEIL1, and MUTYH proteins in conditions of oxidative stress induced by antimycin A (at final concentrations of 10 μM and 100 μM). The control for the Sirt3 silenced and overexpression groups are indicative of the protein level with respect to the wild type. The percentage level is the change in the protein level after a given amount of time with respect to the control. The statistically significant difference () between the increase/decrease in protein level with respect to the wild-type is shown in bold.


Sirt3			10 μM				100 μM
Sirt3			Incubation time (hours)				Incubation time (hours)

		Control	1	3	24	48	1	3	24	48
Sirt3 wild type	Total protein	1,000	0,854	1,453	2,035	2,036	1,235	1,935	1,834	2,135
	Total protein	100%	85%	145%	203%	204%	124%	193%	183%	213%
	Mitochondrial protein	1,000	0,977	0,830	0,882	0,957	0,924	0,661	0,703	0,933
	Mitochondrial protein	100%	98%	83%	88%	96%	92%	66%	70%	93%
Sirt3 silenced	Total protein	0,123	0,154	0,093	0,123	0,082	0,125	0,084	0,125	0,093
	Total protein	12%	125%	76%	100%	67%	102%	69%	101%	76%
	Mitochondrial protein	0,111	0,154	0,094	0,152	0,135	0,124	0,098	0,095	0,152
	Mitochondrial protein	11%	139%	85%	137%	121%	112%	88%	86%	137%
Sirt3 overexpressed	Total protein	3,347	3,457	4,123	4,557	4,214	3,944	4,255	4,856	3,745
	Total protein	335%	103%	123%	136%	126%	118%	127%	145%	112%
	Mitochondrial protein	3,487	4,346	4,463	5,395	6,582	4,568	5,136	6,236	6,782
	Mitochondrial protein	349%	125%	128%	155%	189%	131%	147%	179%	195%

APE1			10 μM				100 μM
APE1			Incubation time (hours)				Incubation time (hours)

		Control	1	3	24	48	1	3	24	48
Sirt3 wild type	Total protein	1,000	1,128	1,806	3,094	3,112	1,472	3,226	3,452	4,843
	Total protein	100%	113%	181%	309%	311%	147%	323%	345%	484%
	Mitochondrial protein	1,000	0,923	1,555	1,635	1,926	1,298	1,734	1,966	2,413
	Mitochondrial protein	100%	92%	155%	163%	193%	130%	173%	197%	241%
Sirt3 silenced	Total protein	0,989	0,777	0,673	1,428	1,508	0,829	0,951	1,507	1,765
	Total protein	98,9%	79%	68%	144%	152%	84%	96%	152%	178%
	Mitochondrial protein	0,973	0,857	1,076	1,529	1,546	0,896	1,215	1,503	1,451
	Mitochondrial protein	97,3%	88%	111%	157%	159%	92%	125%	155%	149%
Sirt3 overexpressed	Total protein	0,979	2,235	3,346	4,123	3,456	2,856	4,123	3,890	4,546
	Total protein	97,9%	228%	342%	421%	353%	292%	421%	397%	464%
	Mitochondrial protein	1,023	2,445	4,472	5,214	4,475	3,123	4,568	4,685	5,789
	Mitochondrial protein	102,3%	239%	437%	510%	437%	305%	447%	458%	566%

NEIL1		10 μM				100 μM
NEIL1		Incubation time (hours)				Incubation time (hours)

	Control	1	3	24	48	1	3	24	48
Sirt3 wild type	Total protein	1,000	1,630	3,185	4,266	5,164	3,023	4,291	5,840	4,810
	Total protein	100%	163%	319%	427%	516%	302%	429%	584%	481%
	Mitochondrial protein	1,000	0,746	0,868	2,439	3,783	0,895	0,728	3,682	3,997
	Mitochondrial protein	100%	75%	87%	244%	378%	90%	73%	368%	400%
Sirt3 silenced	Total protein	1,011	0,744	1,173	1,048	1,388	0,835	0,878	1,277	1,599
	Total protein	101,1%	74%	116%	104%	137%	83%	87%	126%	158%
	Mitochondrial protein	0,981	1,161	1,602	1,744	1,880	1,258	1,712	1,971	2,124
	Mitochondrial protein	98,10%	118%	163%	178%	192%	128%	174%	201%	216%
Sirt3 overexpressed	Total protein	1,024	2,765	4,429	4,672	5,448	4,236	5,234	5,577	5,134
	Total protein	102,4%	270%	433%	456%	532%	414%	511%	545%	501%
	Mitochondrial protein	1,008	1,424	2,343	3,458	4,294	1,235	2,658	4,768	4,238
	Mitochondrial protein	100,8%	141%	232%	343%	426%	122%	264%	473%	420%

MUTYH		10 μM				100 μM
MUTYH		Incubation time (hours)				Incubation time (hours)

	Control	1	3	24	48	1	3	24	48
Sirt3 wild type	Total protein	1,000	1,934	3,124	5,356	5,346	2,345	5,123	6,367	6,235
	Total protein	100%	193%	312%	536%	535%	235%	512%	637%	623%
	Mitochondrial protein	1,000	1,034	0,973	2,457	3,035	1,143	1,043	2,736	3,344
	Mitochondrial protein	100%	103%	97%	246%	303%	114%	104%	274%	334%
Sirt3 silenced	Total protein	1,031	0,893	1,346	2,545	1,923	0,864	1,745	2,134	2,598
	Total protein	103,1%	87%	131%	247%	187%	84%	169%	207%	252%
	Mitochondrial protein	0,981	1,346	2,542	3,125	2,346	1,735	2,124	2,434	2,643
	Mitochondrial protein	98,1%	137%	259%	319%	239%	177%	217%	248%	269%
Sirt3 overexpressed	Total protein	1,023	2,976	4,824	5,748	6,046	3,624	5,975	6,235	6,105
	Total protein	102,3%	291%	472%	562%	591%	354%	584%	609%	597%
	Mitochondrial protein	1,018	1,903	2,437	3,236	3,935	2,454	2,235	3,955	4,144
	Mitochondrial protein	101,8%	187%	239%	318%	386%	241%	219%	388%	407%