Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology

Figure 1

Expression and purification of Tn5 protein. (a) Ptxb1-Tn5 plasmid identification. M Marker; line 1: plasmid digested by Xbal/BamHI. (b) Expression and purification of Tn5 protein by SDS-PAGE electrophoresis, wild-type Tn5 and higher-molecular weight Tn5-intein-CBD is seen above. M Marker; line 1: crude lysate before IPTG induction; line 2: sonication supernatant; line 3: flow-through; line 4: elution after magnetic beads; line 5: DTT eluted fractions.

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