TLR-8, TNF-α, and ESR-1α Gene Polymorphism Susceptibility in Onset of Arthritis

Table 1

PCR-RFLP materials and conditions with respective polymorphic sites.

Gene	SNP ID	F.P	R.P	A.T (^oC)	Product size (bp)	RFLP Enzyme	I.T (^oC)	T.D.T (^oC)	Fragments size (bp)
TLR-8	rs3764879	GTGTGTGTCTGATTTGGGTTG	TTTCTAGGCTCACACCATTTG	58	390	HpyCh4IV	65	—	CC: 390; CG: 390, 228, 162; GG:228, 162
	rs3764880					NlaIII	37	65	AA: 39; AG: 390, 291, 990; GG: 291, 99
	rs5744080	CCCCAAATACCCTCTGGTTT	ATTGAAGCACCACCATCACA	58	416	HpyCh4IV	65	—	CC: 416; CT: 416, 234, 182; TT: 234, 182
TNF	Rs1800629	AGGCAATAGGTTTTGAGGGGCAT	ATCTGGAGGAAGCGGTAGTG	59.5	226	NcoI	37	65	AA: 226; AG: 226, 202; GG: 202
	rs361525					MspI	37	65	GG: 226
ESR-1α	rs2234693	CTGCCATCTTTTCCTATTCTCC	TCTTTCTCTGCCACCCTGGCGTCGATTATTATCTGA	59	636	PuvII	37	80	CC: 636
	rsS9340779					XbaI	37	65	CC: 636
	rs2228480	GTGGAGGAGACGGACCAAA	TGGCCACTCATCTAGAAAGCC	60.5	246	BtgI	37	80	AA: 246; AG: 246, 145, 101; GG: 145, 101
	rs1451501590					—	—		—

F.P: forward primer; R.P: reverse primer; A.T: annealing temperature; RFLP: restriction fragment length polymorphism; I.T: incubation temperature; T.D.T: thermal deactivation temperature.