Molecular Characterization of Wild and Cultivated Strawberry (Fragaria × ananassa) through DNA Barcode Markers

Table 2

Conditions of the selected markers and their sequences used in the current molecular study.

Sr.No	Primers	Primer name	Sequence	Melting temperature (°C)	References
1	ITS2-F; ITS2-R	ITS-S2F; ITS-S3R	5′ATGCGATACTTGGTGTGAAT 3′; 5′GACGCTTCTCCAGACTACAAT 3′	52	[17]
2	ITS-F; ITS-R	ITS-F; ITS-R	5′GCCGTTAAGACCAGGGAT 3′; 5′TGATTCACGGGATTCTGC 3′	50	[18]
3	rbcl-F; rbcL-R	1f; 724r	5′ATGTCACCACAAACAGAAAC3′; 5′TCGCATGTACCTGCAGTAGC 3′	56	[19]
4	rbclc-F; rbCIC-R	HRM_rbcLCF; HRM_rbcLCR	5′TAGACCTTTTTGAAGAAGGTTCTGT3′; 5′TGAGGCGGRCCTTGGAAAGTT 3′	76	[20]
5	rbclA-; FrbclA-R	a-f; a-r	5′ATGTCACCACAAACAGAGACTAAAGC 3′; 5′CTTCTGCTACAAATAAGAATCGATCTC 3′	63	[21]
6	matK-F; MatK-R	2.1a forward; 3.2 reverse	5′ATCCATCTGGAAATCTTAGTTC 3′; 5′ CTTCCTCTGTAAAGAATTC 3′	51	[22]
7	trnV-F; trnV-R	trnV_UAC-E1F; trnV_UAC-E1R	5′ GTAGAGCACCTCGTTTACAC 3′; 5′GTGTAAACGAGGTGCTCTAC 3′	56	[23]
8	ycf3-F; ycf3-R	ycf3-E2F; ycf3-E2R	5′GGCCGTGATCTGTCATTAC3′; 5′TTCCGCGTAATTTCCTTC3′	53.2	[23]