ABIN1 Inhibits Inflammation through Necroptosis-Dependent Pathway in Ulcerative Colitis

Figure 4

Nec-1s mitigate the necroptosis-associated protein expression and intestinal damage in vivo and in vitro. (a) mRNA expression level of ABIN1 in the DSS-induced colitis model; (b) the body weight and DAI index change in mice on combination treatment with DSS, siABIN1 and Nec-1s for 7 days; (c) mRNA expression level of proinflammatory cytokines under ABIN1 ablation and Nec-1s treatment in CACO-2; (d) necroptosis-associated proteins detection expression level under ABIN1 ablation and Nec-1s treatment in CACO-2; and (e) representative images of four groups’ colonic tissue stained with hematoxylin and eosin (H&E; upper) are shown. The bar represents 50 μm and two-tailed student’s t-test was performed to assess statistical significance. , .

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