Genomic and Computational Analysis of Novel SNPs in TNP1 Gene Promoter Region of Bos indicus Breeding Bulls

Figure 1

TNP1 genomic DNA sequences and primers for PCR amplification and sequencing. The recognition site nucleotide sequences, i.e., TATA box nucleotide, are highlighted. Shadowed letters represent single SNPs, while minor alleles are noted beneath the nucleotide. The transcription start site is shown with an asterisk. The arrows above and below the DNA sequence represent the primers employed for polyA-additional signal PCR amplification (the polyA signal from the straight arrow is added to the TNP1 mRNA). Yellow areas represent axons in the TNP1 gene.