Genetic Diversity and Population Structure of Selected Ethiopian Indigenous Cattle Breeds Using Microsatellite Markers

Table 1

Microsatellite markers, their sequence, and chromosomes number location and annealing AT°C using touchdown PCR.

S/n	Loci	Chro	Forward	Reverse	Repeats	AT°C	Size
1	BM1818	23	AGC​TGG​GAA​TAT​AAC​CAA​AGG	AGT​GCT​TTC​AAG​GTC​CAT​GC	(GT)13	67.9–52.9	248–276
2	BM1824	1	GAG CAAGGT GTT TTTCCAATC	CAT​TCT​CCA​ACT​GCT​TCC​TTG	(TG)26	68.4–53.4	170–218
3	BM2113	2	GCTGCCTTCTAC CAA ATA CCC	CTT​CCT​GAG​AGA​AGC​AAC​ACC	(CA/GT)20	71.3–56.3	116–146
4	CSRM60	10	AAG​ATG​TGA​TCC​AAG​AGA​GAG​GCA	AGG​ACC​AGA​TCG​TGA​AAG​GCA​TAG	(CA)17	74.4–59.4	79–115
5	CSSM66	14	AAT​TTA​ATG​CAC​TGA​GGA​GCT​TGG	ACA​CAA​ATC​CTT​TCT​GCC​AGC​TGA	(AC)17	74.4–59.4	171–209
6	ETH10	5	GTT​CAG​GAC​TGG​CCC​TGC​TAA​CA	CCT​CCA​GCC​CAC​TTT​CTC​TTC TC	(CA)12	76.4–61.4	198–234
7	ETH225	9	GAT​CAC​CTT​GCC​ACT​ATT​TCC​T	GTG​TCT​TAC​ATG​ACA​GCC​AGC​TGC​TAC T	(CG)n(TG)4(CA)18	75.2–60.2	132–166
8	ETH3	19	GAA​CCT​GCC​TCT​CCT​GCA​TTG​G	GTG​TCT​TAC​TCT​GCC​TGT​GGC​CAA​GA GG	(GT)26	79–64	90–135
9	ILST006	7	TGTCTGTATTTC TGCTGTGG	ACACGGAAGCGA TCTAAACG	GT)23	67.4–52.4	277–309
10	NRA023	3	GAG​TAG​AGC​TAC​AAG​ATA​AAC​TTC	TAA​CTA​CAG​GGT​GTT​AGA​TGA​ACT​C	(AC)	71.3–56.3	194–236
11	RM067	4	TGA​GTA​ATG​CAA​TAG​ATA​CAG​TAT​T	GCT​TTG​GCC​ATA​TGA​AGA​GCT​TT	(AC)17	69.3-54-3	83–101
12	SPS115	15	AAA​GTG​ACA​CAA​CAG​CTT​CTC​CAG	GTG​TCT​TAA​CGA​GTG​TCC​TAG​TTT​GGC​TGT​G	(CA)27(TA)n	77.9–62.9	240–270
13	TGLA12	21	AAT​CAC​ATG​GCA​AAT​AAG​TAC​ATA​C	CCC​TCC​TCC​AGG​TAA​ATC​AGC	(AC)n(AT)n	71.3–56.3	133–103
14	TGLA16	20	CTA​ATT​TAG​AAT​GAG​AGA​GGC​TTC​T	TTG​GTC​TCT​ATT​CTC​TGA​ATA​TTC​C	(TG)	65.9–55.9	104–133
15	TGLA27	18	GGA​ATT​CCA​AAT​CTG​TTA​ATT​TGC​T	ACA​GAC​AGA​AAC​TCA​ATG​AAG​CA	(TG)n	69.7–54.7	63–115
16	TGLA53	16	GCT​TTC​AGA​AAT​AGT​TTG​CAT​TCA	TGT​CTT​ATC​TTC​ACA​TGA​TAT​TAC​AGC​A GA	(TG)6(CG)4(TA)	71.8–56.8	147–197