Clinical Significance of NKD Inhibitor of WNT Signaling Pathway 1 (NKD1) in Glioblastoma

Figure 4

NKD1 is correlated with immune cell enrichment and inhibits GBM growth. (a) RT-qPCR method was used to validate transfection efficiencies of pcDNA3-NKD1 and pcDNA3-vector plasmids. The blank group referred to cells treated with only transfection reagent without any plasmid. (b) Colony formation assay was conducted to evaluate GBM cell proliferation capacity. (c) The MTT method was used to dynamically monitor the growth curves of transfected GBM cells. (d) The correlations between immune cell enrichment and NKD1-mRNA level in GBM tissues were summarized. (e) Spearman correlation test indicated a negative correlation between NKD1-mRNA level and T cell infiltration in GBM.

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