Anti-Early Stage of Bacterial Recolonization Effect of Curcuma longa Extract as Photodynamic Adjunctive Treatment

Table 1

Target primer/probe sequence and amplification condition for quantitative real-time PCR used in this study.

	Primer sequence (5′-3′)	Thermal program
Prevotella intermedia	F : CCACATATGGCATCTGACGTG	10 min at 95^°C, 40 cycles at 95^°C for 15 s, 58^°C for 60 s, 72^°C for 45 s, and a final extension at 72^°C for 5 min
	R : TCAATCTGCACGCTACTTGG
	P : FAM-ACCAAAGATTCTACGGTGGAGGATGGG-QSY
Fusobacterium nucleatum	F : CGCAGAAGGTGAAAGTCCTGTAT
	R : TGGTCCTCACTGATTCACACAGA
	P : ABY- ACTTTGCTCCCAAGTAACATGGAACAC GAG-QSY

F: forward primer; R: reverse primer; P: probe; FAM: 6-carboxyfluorescein.