Research Article
Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study
Figure 5
Relative gene expression quantitation during differentiation: quantification of the relative expression of specific osteoblasts and mineralizing transcripts runt-related transcription factor 2 (RUNX2), Osterix (OSX), and Osteocalcin (OCN) was performed in hDPSCs treated with vitamins D, E, and D + E during 7, 14, and 21 days. Data is expressed in relation to the expression levels of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene and as a positive control, and cells treated with differentiation medium (DM) were analyzed.