Effects of Chronic Ethanol Consumption and Ovariectomy on the Spontaneous Alveolar Bone Loss in Rats

Figure 1

Study design. First, the female Wistar rats were ovariectomized at 75 days of age. After 15 days of the procedure, distilled water–H₂O_dist (Control group; OVX group) or ethanol–EtOH (EtOH group; EtOH + OVX) were administered by intragastric gavage during 55 days. At the end of the administration, the animals (145 days old) were euthanized. Their hemimandibles were collected. Afterwards, they were stained with methylene blue allowing the measurement of alveolar bone loss with stereomicroscope.