Desquamated Epithelial Cells of Unstimulated Human Whole Saliva Express Both EGF Transcript and Protein

Figure 1

EGF immunoreactivity is detected in cell lysates of DOECs by using dot blot. Cells were isolated from human unstimulated whole saliva, lysed in SDS sample buffer, sonicated, boiled, and then the cell lysates were centrifuged, and the supernatants were collected for analysis. EGF immunoreactivity was assessed in samples prepared from three of the four individuals (1, 2, and 3) included in the study. Each sample was analyzed in duplicate. SDS sample buffer was utilized as blank.