Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-β1 Liberation from Dentin In Vitro

Figure 4

The effects of acetylsalicylic acid (ASA) treatment enhanced the odontogenic capacity of human dental pulp cells (HDPCs) in a dose-dependent manner. (a, b) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) demonstrated the dentin sialophosphoprotein (DSPP) and runt-related nuclear factor 2 (RUNX2) gene expression levels of HDPCs at 14 d of odontogenic induction (n = 6). Results showed the ratios to the control. (c-e) Alkaline phosphatase (ALP), dentin sialoprotein (DSP), and RUNX2 biomarker levels were evaluated after 7, 14, and 21 d of odontogenic induction (n = 4). (f) Alizarin red staining showed that high concentrations of ASA significantly promoted the mineralization of HDPCs after 7, 14, and 21 d of treatment (n = 6). The biomarker and alizarin red levels were normalized to cell numbers per million cells showing that the production per cell decreased over time. . . Values are presented as mean ± SD.

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