Effect of Long-Term Cryopreservation on the Stemness of Stem Cells of Apical Papilla

Table 2

Primer design for Q-RTPCR (forward 5′-3 and reversed 5′-3′ sequences for molecular analysis).

Gene	Forward 5′-3′	Reversed 5′-3′
ZNF217	GGAATGGAACAACAGCGG	AAA​CAT​GCC​AAC​TCA​ATC​CCT
ZNF878	GAA​ATA​AAT​CCC​TTA​CCA​CA	ACC​CTA​TGA​GTG​TAC​ACA​AT
ESRRB	CCC​ACT​TTG​AGG​CAT​TTC​AT	ACA​TTG​CCT​CTG​GCT​ACC​AC
SALL4	AGT​ACA​GCT​CCG​GAG​AAG​TC	AAT​GCT​GTG​CGG​AGT​TCT​TC
KLF4	CCG​CCA​GCG​GTT​ATT​CGG​GG	GCA​GCC​ACC​TGG​CGA​GTC​TG
SOX2	CGA​GCT​GGT​CAT​GGA​GTT​GTA	TAC​AGC​ATG​ATG​CAG​GAC​CA
NANOG	TGC​TGG​AGG​CTG​AGG​TAT​TTC​TGT​CTC	AGT​CCC​AAA​GGC​AAA​CAA​CCC​ACT​TC
OCT4	TGG​GAC​TCC​TCC​GGG​TTT​TG	AGC​CCT​CAT​TTC​ACC​AGG​CC
CMYC	GCA​CAA​GAG​TTC​CGT​AGC​TG	AAA​GGC​CCC​CAA​GGT​AGT​TA
GAPDH	ACG​GCA​GGT​CAG​GTC​CAC​CA	ACT​GTG​GAT​GGC​CCC​TCC​GG