Prolonged Inhibition of Streptococcus mutans Growth and Biofilm Formation by Sustained Release of Chlorhexidine from Varnish Coated Dental Abutments: An in Vitro Study

Figure 2

Kinetics of bacterial growth inhibition. Abutments 1, 2, and 3 coated with SRV-CHX (1-SRV-CHX, 2-SRV-CHX, and 3-SRV-CHX) and SRV-placebo were placed on agar plates pre-seeded with 50 microliters S. mutans at OD₅₉₅ = 0.02. The plates were incubated at 37°C aerobically with 5% CO₂. The zone of inhibition (ZOI; cm²) around the placed abutments was measured daily, and the abutments were removed aseptically and placed on new S. mutans pre-seeded plates for further incubations. (a) Quantitative analysis of bacterial growth inhibition by SRV-CHX-coated abutments [1–3]. ^∗Significantly higher than the value for SRV-placebo (). (b) Images of inoculated agar plates after 21 days of incubation with an inserted 1-, 2-, or 3-SRV-CHX/placebo-coated abutments.

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