Equisetum arvense Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis

Figure 4

Effects of EA on the LPS induced NF-κB and MAPKs’ signaling pathway in ST2. (a) E. coli-LPS (1 μg/mL) is applied to ST2 cells and collected at several time points. NF-κB p65 and MAPKs (JNK and p38) in ST2 cells are analyzed by Western blotting. β-actin is used as a loading control. (b) EA: Equisetum arvense extract (3 μg/mL) is pretreated in ST2 cells for 30 minutes, and then E. coli-LPS (1 μg/mL) is additionally applied to ST2 cells. IKKα/β, NF-κB p65, and MAPKs (JNK and p38) in ST2 cells are assessed at 30 minutes by Western blotting. β-actin is used as the loading control.

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