Anti-RANKL Inhibits Thymic Function and Causes DRONJ in Mice

Figure 3

Immunochemical examination and TUNEL assay of the thymus. (a) The upper panels show an immunohistochemical image in the thymus with isotype IgG, which is used as a negative control of IgG and anti-RANKL/Mel. The middle panels show immunohistochemistry of a low magnification image in the thymus stained with anti-AIRE of IgG and anti-RANKL/Mel. The lower panels show immunohistochemistry of a high magnification image in the thymus stained with anti-AIRE of IgG and anti-RANKL/Mel. Black arrows point to the AIRE positive cells. Bar is 100 μm. (b) The upper panels show TUNEL reaction of a low magnification image in the thymus of IgG and anti-RANKL/Mel. Bar is 100 μm. The middle panels show TUNEL reaction of a high magnification image in the cortex of IgG and anti-RANKL/Mel. The lower panels show TUNEL reaction of a high magnification image in the medulla of IgG and anti-RANKL/Mel. Bar is 50 μm. (c) Number of TUNEL positive cells in the cortex and medulla of IgG vs. anti-RANKL/Mel. Graphs show mean ± SEM. .

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