Anti-RANKL Inhibits Thymic Function and Causes DRONJ in Mice

Figure 4

Flow cytometry analysis of thymocytes, PBMCs, and splenocytes. (a) Flow cytometric analysis of thymocytes from IgG and anti-RANKL/Mel. Thymocytes were analyzed for CD4 and CD8 expression. The numbers indicate the percent of total thymocytes that fall within the indicated quadrants. (b) Flow cytometric analysis of PBMCs from IgG and anti-RANKL/Mel. Thymocytes were analyzed for CD4 and CD8 expression. The numbers indicate the percent of total thymocytes that fall within the indicated quadrants. (c) Flow cytometric analysis of splenocytes from IgG and anti-RANKL/Me. Splenocytes were analysed for CD4 and CD8 expression. The numbers indicate the percent of total thymocytes that fall within the indicated quadrants. (d) Percent of CD4 SP, CD8 SP, and CD4CD8 DP cells in IgG and anti-RANKL/Mel. (e) Percent of CD4 SP, CD8 SP, and CD4CD8 DP cells in IgG and anti-RANKL/Mel mice. (f) Percent of CD25 and FoxP3 positive Treg cells in and anti-RANKL/Mel. Graphs show mean ± SEM. .

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