“The Standard Procedure” for Investigation of Oral Neutrophils in Oral Diseases

Figure 1

Effect of fixation, storage temperature, and duration of storage on the concentration of neutrophils in mouth rinse. The mouth rinses were either fixated in 10% formalin or left unfixed and stored at 5°C or room temperature (RT) before preparation and flow cytometrical analysis on day 0, 1, 3, and 7 after collection. Statistically significant decline in number of neutrophils per milliliter in nonfixed samples despite of storage temperature when comparing to day 0. Statistical analysis by two-way ANOVA. .