Anti-skin Aging Potential of Sargassum thunbergii Ethanolic Extract: Antioxidant, Anti-inflammatory, and Antiwrinkle Effects on L929 Fibroblast Cells

Figure 8

Toxicity assessment of STEE: (a) Cell viabilities of the 3D human skin model EpiSkin™ in the skin irritation test. An MTT assay was performed on EpiSkin™ samples treated with STEE for 15 min and postincubated for 42 h. NC and PC were treated with H₂O or 5% SDS, respectively; (b) the hemolytic activity of STEE on horse erythrocytes. The 100% hemolysis was conducted using the treatment of the positive control, 0.1% Triton X-100. The erythrocytes that was resuspended in PBS was used as negative control.

(a)

(b)