Prevalence and Phenotypic and Genotypic Patterns of Antibiotic Resistance of Acinetobacter baumannii Strains Isolated from Fish, Shrimp, and Lobster Samples

Table 1

Conditions used for the PCR-based detection of antibiotic resistance genes in the A. baumannii isolates [23–26].

Target gene	Primer (5-3)	Size (bp)	PCR cycles	PCR volume
aadA1	(F) TATCCAGCTAAGCGCGAACT; (R) ATTTGCCGACTACCTTGGTC	447	1 cycle: 94°C for 6 min; 33 cycles: 95°C for 70 s, 55°C for 65 s, and 72°C for 90 s; 1 cycle: 72°C for 8 min	5 μL of PCR buffer 10x; 2 mM of Mgcl2; 150 μM of dNTP; 1 μM of each primer (F and R); 1 U of Taq DNA polymerase; 3 μL of DNA template
aac(3)–IV	(F) CTTCAGGATGGCAAGTTGGT; (R) TCATCTCGTTCTCCGCTCAT	286
sul1	(F) TTCGGCATTCTGAATCTCAC; (R) ATGATCTAACCCTCGGTCTC	822
blaSHV	(F) TCGCCTGTGTATTATCTCCC; (R) CGCAGATAAATCACCACAATG	768
blaCITM	(F) TGGCCAGAACTGACAGGCAAA; (R) TTTCTCCTGAACGTGGCTGGC	462
cat1	(F) AGTTGCTCAATGTACCTATAACC; (R) TTGTAATTCATTAAGCATTCTGCC	547
cmlA	(F) CCGCCACGGTGTTGTTGTTATC; (R) CACCTTGCCTGCCCATCATTAG	698
tetA	(F) GGTTCACTCGAACGACGTCA; (R) CTGTCCGACAAGTTGCATGA	577
tetB	(F) CCTCAGCTTCTCAACGCGTG; (R) GCACCTTGCTGATGACTCTT	634
dfrA1	(F) GGAGTGCCAAAGGTGAACAGC; (R) GAGGCGAAGTCTTGGGTAAAAAC	367
qnrA	(F) GGGTATGGATATTATTGATAAAG; (R) CTAATCCGGCAGCACTATTTA	670
blaIMP	(F) GAATAGAATGGTTAACTCTC; (R) CCAAACCACTAGGTTATC	188	1 cycle: 95°C for 4 min; 30 cycles: 95°C for 45 s, 58°C for 60 s, and 72°C for 40 s; 1 cycle: 72°C for 5 min	5 μL of PCR buffer 10x; 2 mM of Mgcl2; 150 μM of dNTP; 1 μM of each primer (F and R); 1 U of Taq DNA polymerase; 3 μL of DNA template
blaVIM	(F) GTTTGGTCGCATATCGCAAC; (R) AATGCGCAGCACCAGGATAG	382
blaSIM	(F) GTACAAGGGATTCGGCATCG; (R) GTACAAGGGATTCGGCATCG	569
blaOxa-51-like	(F) TAATGCTTTGATCGGCCTTG; (R) TGGATTGCACTTCATCTTGG	353	1 cycle: 94°C for 5 min; 32 cycles: 95°C for 50 s, 60°C for 60 s, and 72°C for 70 s; 1 cycle: 72°C for 10 min	5 μL of PCR buffer 10x; 2 mM of Mgcl2; 150 μM of dNTP; 1 μM of each primer (F and R); 1 U of Taq DNA polymerase; 3 μL of DNA template
blaOxa-23-like	(F) GATCGGATTGGAGAACCAGA; (R) ATTTCTGACCGCATTTCCAT	501
blaOxa-24-like	(F) GGTTAGTTGGCCCCCTTAAA; (R) AGTTGAGCGAAAAGGGGATT	246
blaOxa-58-like	(F) AAGTATTGGGGCTTGTGCTG; (R) CCCCTCTGCGCTCTACATAC	599