Optimization of Conjugated Linoleic Acid and Eicosapentaenoic Acid Production from Sesame Waste by Response Surface Methodology

Table 1

Independent variables and response data in the response surface methodology (RSM) method.

Real	Value			Response 1; pH	Response 2; Absorbance
Run
1	37 (0)	2 (-1)	60 (-1)	4.98	0.65
2	30 (-1)	4 (0)	60 (-1)	4.8	0.7
3	37 (0)	4 (0)	70 (0)	4.82	0.8
4	44 (+1)	2 (-1)	70 (0)	5.2	0.2
5	30 (-1)	4 (0)	80 (+1)	4.95	2.5
6	37 (0)	4 (0)	70 (0)	4.83	0.43
7	37 (0)	4 (0)	70 (0)	4.82	0.3
8	37 (0)	2 (-1)	80 (+1)	4.9	1.5
9	37 (0)	6 (+1)	60 (-1)	4.97	0.5
10	37 (0)	4 (0)	70 (0)	4.85	0.47
11	30 (-1)	2 (-1)	70 (0)	4.98	0.9
12	44 (+1)	4 (0)	60 (-1)	5.2	0.2
13	37 (0)	4 (0)	70 (0)	4.75	0.4
14	37 (0)	6 (+1)	80 (+1)	4.7	2.5
15	44 (-1)	4 (0)	80 (+1)	4.8	0.3
16	30 (-1)	6 (+1)	70 (0)	5	0.8
17	44 (+1)	6 (+1)	70 (0)	5	0.3

: temperature; : inoculation; : humidity. Average and standard deviation for pH and absorbance are and , respectively.