Research Article
The Identification of Yak Meat Using Loop-Mediated Isothermal Amplification Method Coupled with Hydroxy Naphthol Blue for the Prevention of Food Fraud
Table 2
Optimal system of 25 μL for LAMP detection assay.
| | Reagents | Concentration | Volume (μL) |
| | 10x buffer | — | 2.5 | | DNTP | 1 mmol/L | 2.5 | | MgSO4 | 5 mmol/L | 3 | | FIP | 1200 mmol/L | 3 | | BIP | 1200 mmol/L | 3 | | F3 | 400 mmol/L | 1 | | B3 | 400 mmol/L | 1 | | Bst DNA polymerase | 0.8 U/μL | 1 | | HNB | 0.15 mmol/L | 1.5 | | ddH2O | — | Up to 25 | | Sample DNA solution | — | 5 |
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