Adjusting Effects of Baicalin for Nuclear Factor-<mml:math id="E1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>κ</mml:mi></mml:math>B and Tumor Necrosis Factor-<mml:math id="E2" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math> on Rats With Caerulein-Induced Acute Pancreatitis

Xue, Dongbo; Zhang, Weihui; Zhang, Yingmei; Wang, Haiyang; Zheng, Biao; Shi, Xingye

doi:https://doi.org/10.1155/MI/2006/26295

Mediators of Inflammation

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Research Communication | Open Access

Volume 2006 | Article ID 026295 | https://doi.org/10.1155/MI/2006/26295

Adjusting Effects of Baicalin for Nuclear Factor-κB and Tumor Necrosis Factor-α on Rats With Caerulein-Induced Acute Pancreatitis

Dongbo Xue,¹Weihui Zhang,¹Yingmei Zhang,²Haiyang Wang,¹Biao Zheng,¹and Xingye Shi¹

Received27 Apr 2006

Revised23 Jul 2006

Accepted07 Aug 2006

Published19 Sept 2006

Abstract

Forty Wistar rats were divided into 5 groups, including the control group, the acute pancreatitis group (AP group, induced by intraperitoneal injections of caerulein), and the AP group treated with baicalin, the AP group treated with LPS, and the AP group treated with LPS and baicalin. Pathological damage of pancreatic tissue was scored with hematoxylin and eosin (HE) staining. The mRNA expression of TNF-α was measured with semiquantitative RT-PCR, and activation of NF-κB was detected with flow cytometry assay. It was shown in the results that the expression of TNF-α mRNA, activation of NF-κB, and pathological score of AP group were all obviously higher than those of control group (P<.01). In AP group treated with LPS, further rise of these values were observed (P<.01). In the AP group treated with baicalin, activation of NF-κB decreased (P<.05), and expression of TNF-α mRNA also obviously decreased (P<.01), while pancreatic pathological damage was alleviated at the same time (P<.01); similar results were observed in AP group treated with LPS and baicalin (P<.01), which indicated that baicalin might be applied to inhibit NF-κB activating and TNF-α expressing so as to treat AP.

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Copyright © 2006 Dongbo Xue et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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