The Relationship Between Serum Levels of Total I<small.letters>g</small.letters>E, IL-18, IL-12, IFN-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>γ</mml:mi></mml:math> and Disease Severity in Children With Atopic Dermatitis

Aral, Murat; Arican, Ozer; Gul, Mustafa; Sasmaz, Sezai; Kocturk, Sumeyra Alkis; Kastal, Ummugulsum; Ekerbicer, Hasan Cetin

doi:https://doi.org/10.1155/MI/2006/73098

Mediators of Inflammation

On this page

Abstract References Copyright Related Articles

Research Communication | Open Access

Volume 2006 | Article ID 073098 | https://doi.org/10.1155/MI/2006/73098

The Relationship Between Serum Levels of Total IgE, IL-18, IL-12, IFN-γ and Disease Severity in Children With Atopic Dermatitis

Murat Aral,¹Ozer Arican,²Mustafa Gul,¹Sezai Sasmaz,²Sumeyra Alkis Kocturk,¹Ummugulsum Kastal,²and Hasan Cetin Ekerbicer³

Received11 Nov 2005

Revised22 Mar 2006

Accepted22 Mar 2006

Published11 May 2006

Abstract

Studies about the role of cytokines on the immunopathogenesis of atopic dermatitis (AD) are generally based on in vitro observations and this role has not been completely clarified yet. Serum levels of total IgE, IL-18, IL-12, IFN-γ and the relationship between these parameters and disease severity, determined using the SCORAD index, in a group of atopic patients were investigated in this study. Serum levels of total IgE were measured by the nephelometric method and serum levels of IL-18, IL-12/p40 and IFN-γ were measured by ELISA method. Serum levels of total IgE and IL-18 were found significantly higher in study group than in controls (P<.001). There was no statistically significant difference between patients and controls in respect of serum levels of IL-12/p40 (P=.227). A statistically significant relationship between SCORAD values and serum levels of total IgE (P<.001), IL-18 (P<.001), and IL-12/p40 (P<.001) was determined. These results show that serum levels of IL-18 can be a sensitive parameter that importantly correlates with clinical severity of AD, can play a role in the immunopathogenesis of AD, and furthermore may be used in the diagnosis and follow-up of the disease in addition to other parameters.

References

A R Halbert, W L Weston, and J G Morelli, “Atopic dermatitis: is it an allergic disease?,” Journal of the American Academy of Dermatology, vol. 33, no. 6, pp. 1008–1018, 1995.
View at: Google Scholar
J M Hanifin, “Atopic dermatitis,” Journal of Allergy and Clinical Immunology, vol. 73, no. 2, pp. 211–222, 1984.
View at: Google Scholar
D YM Leung and Q Hamid, “The immunology of atopic dermatitis,” in Atopic Dermattis: From Pathogenesis to Treatment, D YM Leung, Ed., p. 111, RG Landes, Austin, Tex, 1995.
View at: Google Scholar
B J Demis, “Atopic dermatitis,” in 19th Rv Clinical Dermatology, D J Demis, Ed., Lippincott, Philadelphia, Pa, 1992; 39: 13-3.
View at: Google Scholar
S J Galli, J R Gordon, and B K Wershil, “Cytokine production by mast cells and basophils,” Current Opinion in Immunology, vol. 3, no. 6, pp. 865–873, 1991.
View at: Google Scholar
K Katagiri, S Itami, Y Hatano, and S Takayasu, “Increased levels of IL-13 mRNA, but not IL-4 mRNA, are found in vivo in peripheral blood mononuclear cells (PBMC) of patients with atopic dermatitis (AD),” Clinical and Experimental Immunology, vol. 108, no. 2, pp. 289–294, 1997.
View at: Google Scholar
Q Hamid, M Boguniewicz, and D YM Leung, “Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis,” Journal of Clinical Investigation, vol. 94, no. 2, pp. 870–876, 1994.
View at: Google Scholar
G Girolomoni, M B Lutz, S Pastore, C U Assmann, A Cavani, and P Ricciardi-Castagnoli, “Establishment of a cell line with features of early dendritic cell precursors from fetal mouse skin,” European Journal of Immunology, vol. 25, no. 8, pp. 2163–2169, 1995.
View at: Google Scholar
R P Schleimer, S A Sterbinsky, J Kaiser et al., “IL-4 induces adherence of human eosinophils and basophils but not neutrophils to endothelium: association with expression of VCAM-1,” Journal of Immunology, vol. 148, no. 4, pp. 1086–1092, 1992.
View at: Google Scholar
M Tanaka, S Aiba, N Matsumura et al., “IgE-mediated hypersensitivity and contact sensitivity to multiple environmental allergens in atopic dermatitis,” Archives of Dermatology, vol. 130, no. 11, pp. 1393–1401, 1994.
View at: Google Scholar
H Okamura, H Tsutsui, T Komatsu et al., “Cloning of a new cytokine that induces IFN- $\gamma$ production by T cells,” Nature, vol. 378, no. 6552, pp. 88–91, 1995.
View at: Google Scholar
H Okamura, H Tsutsui, S-I Kashiwamura et al., “Interleukin-18: a novel cytokine that augments both innate and acquired immunity,” Advances in Immunology, vol. 70, pp. 281–312, 1998.
View at: Google Scholar
T Yoshimoto, K Takeda, T Tanaka et al., “IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: synergism with IL-18 for IFN- $\gamma$ production,” Journal of Immunology, vol. 161, no. 7, pp. 3400–3407, 1998.
View at: Google Scholar
G Trinchieri, “Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity,” Annual Review of Immunology, vol. 13, pp. 251–276, 1995.
View at: Google Scholar
T Hoshino, R H Wiltrout, and H A Young, “IL-18 is a potent coinducer of IL-13 in NK and T cells: a new potential role for IL-18 in modulating the immune response,” Journal of Immunology, vol. 162, no. 9, pp. 5070–5077, 1999.
View at: Google Scholar
T Hoshino, H Yagita, J R Ortaldo et al., “In vivo administration of IL-18 can induce IgE production through Th2 cytokine induction and up-regulation of CD40 ligand (CD154) expression on ${\text{CD}}4^ +$ T cells,” European Journal of Immunology, vol. 30, no. 7, pp. 1998–2006, 2000.
View at: Google Scholar
H Miyajima, T Hirano, S Hirose et al., “Suppression by IL-12 of IgE production by B cells stimulated by IL-4,” Journal of Immunology, vol. 146, no. 2, pp. 457–462, 1991.
View at: Google Scholar
P Jeannin, Y Delneste, M Seveso et al., “IL-12 synergizes with IL-2 and other stimuli in inducing IL-10 production by human T cells,” Journal of Immunology, vol. 156, no. 9, pp. 3159–3165, 1996.
View at: Google Scholar
P T Mehrotra, R P Donnelly, S Wong et al., “Production of IL-10 by human natural killer cells stimulated with IL-2 and/or IL-12,” Journal of Immunology, vol. 160, no. 6, pp. 2637–2644, 1998.
View at: Google Scholar
J M Hanifin and G Rajka, “Diagnostic features of atopic dermatitis,” Acta Dermato-Venereologica. Supplementum, vol. 92, pp. 44–47, 1980.
View at: Google Scholar
J F Stalder, A Taieb, D J Atherton et al., “Severity scoring of atopic dermatitis: the SCORAD index. Consensus Report of the European Task Force on Atopic Dermatitis,” Dermatology, vol. 186, no. 1, pp. 23–31, 1993.
View at: Google Scholar
I Roitt, J Brostoff, and D Male, Immunology, Mosby, Barcelona, Spain, 4th edition, 1996, 21.1-22.13.
View at: Google Scholar
E Johnson, J Irons, and R Patterson, “Serum IgE concentration in atopic dermatitis,” Journal of Allergy and Clinical Immunology, vol. 55, pp. 256–267, 1975.
View at: Google Scholar
M Worm and B M Henz, “Molecular regulation of human IgE synthesis,” Journal of Molecular Medicine, vol. 75, no. 6, pp. 440–447, 1997.
View at: Google Scholar
N Laske and B Niggemann, “Does the severity of atopic dermatitis correlate with serum IgE levels?,” Pediatric Allergy and Immunology, vol. 15, no. 1, pp. 86–88, 2004.
View at: Google Scholar
I B McInnes, J A Gracie, B P Leung, X-Q Wei, and F Y Liew, “Interleukin 18: a pleiotropic participant in chronic inflammation,” Immunology Today, vol. 21, no. 7, pp. 312–315, 2000.
View at: Google Scholar
H Koning, H J Neijens, M RM Baert, A P Oranje, and H FJ Savelkoul, “T cell subsets and cytokines in allergic and non-allergic children. I. Analysis of IL-4, IFN- $\gamma$ and IL-13 mRNA expression and protein production,” Cytokine, vol. 9, no. 6, pp. 416–426, 1997.
View at: Google Scholar
K Shida, H Koizumi, I Shiratori et al., “High serum levels of additional IL-18 forms may be reciprocally correlated with IgE levels in patients with atopic dermatitis,” Immunology Letters, vol. 79, no. 3, pp. 169–175, 2001.
View at: Google Scholar
Y Yoshizawa, H Nomaguchi, S Izaki, and K Kitamura, “Serum cytokine levels in atopic dermatitis,” Clinical and Experimental Dermatology, vol. 27, no. 3, pp. 225–229, 2002.
View at: Google Scholar
K LE Hon, T F Leung, K C Ma, C K Wong, H Wan, and C WK Lam, “Serum concentration of IL-18 correlates with disease extent in young children with atopic dermatitis,” Pediatric Dermatology, vol. 21, no. 6, pp. 619–622, 2004.
View at: Google Scholar
D YM Leung, “Pathogenesis of atopic dermatitis,” Journal of Allergy and Clinical Immunology, vol. 104, no. 3 II, pp. S99–S108, 1999.
View at: Google Scholar

Copyright

Copyright © 2006 Murat Aral et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation Order printed copies

Views

361

Downloads

1056

Citations