Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor B
Figure 2
Effects of
polymyxin B on LP or LPS-induced TNF- production in THP-1 cells. LP or LPS was pretreated by 100 μg/mL polymyxin B (PB)
for 2 hours before challenging THP-1 cells. LP (3 g/mL) or LPS (100 ng/mL) was
added to the medium. After being incubated for 24 hours, cells were harvested,
and the concentration of TNF- in the medium was determined by ELISA as
described in Section 2.