Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor B
Figure 4
The activity of NF-κB in different groups was
examined by EMSA. NF-κB activation was measured
by EMSA using a biotin-labeled oligonucleotide encompassing the NF-κB consensus
motif. THP-1 cells were stimulated with M.
genitalium LP (3 g/mL) at different time
intervals (0, 1, 2, and 4 hour(s)). THP-1 cells treated with 100 L aqueous phase and 0.1 g/mL LPS
were used as control. The specificity of DNA binding was assessed by
preincubating extracts with unlabeled specific (NF-κB) or unspecific (AP-1)
competitor oligonucleotide. The arrow indicates specific NF-κB band.