Mycoplasma genitalium Lipoproteins Induce Human Monocytic Cell Expression of Proinflammatory Cytokines and Apoptosis by Activating Nuclear Factor B
Figure 5
Cell apoptosis of different groups was
detected by Annexin-V-propidium iodide staining. THP-1 cells were stimulated with 3 g/mL of LP, 3 g/mL of LP in
combination with 25 M PDTC, or 0.1 g/mL LPS for 12 hours, stained with
Annexin-V-FITC-PI and analyzed by FACS. Double negative staining represents living
cells (c), positive staining for Annexin-V-FITC, and negative staining
for PI represent the early apoptotic stage (e), and double-positive
staining represents the late apoptotic stage (b).
