Research Article

Neutrophil and Monocyte CD64 and CD163 Expression in Critically Ill Neonates and Children with Sepsis: Comparison of Fluorescence Intensities and Calculated Indexes

Table 3

Optimum diagnostic cutoff level, diagnostic accuracy with 95% confidence interval (CI) determined by the area under the ROC curve (AUC), sensitivity, and specificity for given cutoff levels of CD64 and CD163 MFI and indexes (i) for neutrophils and monocytes and CD64 score points for sepsis prediction in critically ill neonates and children at the time of suspected sepsis (day 0) and 24 hours later (day 1).

Cutoff levelAUC (95% CI)Sensitivity (%)Specificity (%)
Day01010101

CD64MFI for neutrophils72650.854 (0.734–0.934)0.983 (0.885–0.995)65.595.592.695.0
CD64i for neutrophils2.452.190.833 (0.709–0.919)0.975 (0.872–0.996)65.586.488.9100
CD64MFI for monocytes2282660.865 (0.747–0.941)0.858 (0.715–0.946)72.472.788.995.0
CD64i for monocytes8.709.470.811 (0.684–0.903)0.825 (0.677–0.924)65.563.696.395.0
CD163MFI for neutrophils47550.655 (0.516–0.777)0.750 (0.592–0.870)75.986.451.960.0
CD163i for neutrophils3914820.595 (0.455–0.724)0.677 (0.515–0.813)86.277.337.065.0
CD163MFI for monocytes164130330.713 (0.576–0.826)0.682 (0.520–0.817)75.963.674.185.0
CD163i for monocytes19257342820.635 (0.495–0.759)0.500 (0.338–0.654)51.727.388.995.0
CD64MFI score point>0 (1 and 2)>0 (1 and 2)0.879 (0.764–0.951)0.961 (0.851–0.995)86.2100.085.290.0
CD64i score point>0 (1 and 2)>0 (1 and 2)0.864 (0.746–0.941)0.914 (0.785–0.977)82.890.988.980.0