Lymphotoxin- Plays Only a Minor Role in Host Resistance to Respiratory Infection with Virulent Type A Francisella tularensis in Mice
Figure 3
Cytokine and chemokine levels in sera (a)
and bronchoalveolar lavage (BAL) fluid (b) of mice inoculated by aerosol with
type A F. tularensis. Groups of LTα−/− (open symbols) and LTα+/+ (closed symbols) mice (n = 5) were
challenged by aerosol with low-dose type A F.
tularensis strain, FSC033 (inhaled dose of ~10 organisms) on day 0, and blood
samples and bronchoalveolar lavage fluid samples were collected at dpi 0, 2,
and 4. Cytokine and chemokine levels in the serum and BAL fluid were determined
using the Beadlyte Mouse 21-Plex Cytokine Detection System on a Luminex 100 IS
instrument. Each symbol represents the corresponding cytokine concentration of
an individual mouse. Horizontal lines indicate the median of each group of mice
on the indicated post-inoculation days. The detection limits of the assays
were <5 pg/ml for both sera and BAL fluid. versus LTα+/+ mice.
